Measuring at the same position of the images (height information from red line), the membrane hole size was measured as 930nm in the 1^st image. The hole increased in size to 1560nm and then to 3010nm. Finally, as shown in the 4^th image, hole size grew to 3230nm. By calculating the cellular membrane’s morphological changes with respect to imaging time, the membrane’s movement velocity could be analyzed quantitatively, as shown on the right. The greatest velocity of cellular membrane motion was 10.4nm/sec. during the hole size change between the 2^nd and 3^rd images.

Three-dimensional volume calculations of each hole allow measuring volumetric changes in velocity of the cellular membrane, which is only obtainable from a 3D cell image, as taken by SICM. In the 1st image, the volume of the hole was measured as 0.113μm3. The size increased to 0.280μm³ in the next image, from which the velocity of membrane volumetric change was estimated as 0.0012μm³/sec.In the next hole volume change from 2^nd to 3^rd, the velocity was about seven times faster (0.0075μm³/sec.) than during the previous imaging interval.